Engineering and characterization of a single chain surrogate light chain variable domain

Lactobacilli-Expressed Single-Chain Variable

Identification of a Pre-BCR Lacking Surrogate Light Chain

SLP-65-/- pre-B cells show a high proliferation rate in vitro. We have shown previously that lambda5 expression and consequently a conventional pre-B cell receptor (pre-BCR) are essential for this proliferation. Here, we show that pre-B cells express a novel receptor complex that contains a micro heavy chain (microHC) but lacks any surrogate (SL) or conventional light chain (LC). This SL-defici...

production and characterization of recombinant light chain and carboxyterminal heavy chain fragments of tetanus toxin

background: light chain (lc) and heavy chain carboxyterminal subdomain (hcc) fragments are the most important parts of tetanus neurotoxin (tent) which play key roles in toxicity and binding of tent, respectively. in the present study, these two fragments were cloned and expressed in a prokaryotic system and their identity was confirmed using anti-tent specific polyclonal and monoclonal antibodi...

Construction of recombinant Pichia pastoris expressing single-chain antibody fragment against extracellular domain of EpCAM

Introduction: Epithelial cell adhesion molecule (EpCAM) is highly expressed on epithelial tumors. So, EpCAM is a valuable antigen for targeted therapy. Using monoclonal antibodies (mabs) is an attractive approach for targeted cancer therapy. Importantly, limitations of intact mabs including large size led to the development of antibody fragments such as single chain fragment variable (scfv). Pi...

Design and Construction of a Novel Humanized Single-Chain Variable-Fragment Antibody against the Tumor Necrosis Factor alpha

The pro-inflammatory cytokine, TNF-α, which plays a major role in the development and persistence of inflammatory diseases, is the basis for the use of anti-TNF-α therapies. The neutralization of TNF-α or blockage of its binding to the corresponding receptor has mainly served as a therapeutic strategy against some diseases. This study aimed to investigate the production of a humanized single ch...